Saturday, August 10, 2019
Gene Essay Example | Topics and Well Written Essays - 2000 words
Gene - Essay Example will be joined with plasmids cut by the same restriction enzymes through their compatible and matching sticky ends, producing a recombinant DNA molecule, which will be inserted to an appropriate host cell. Plasmids can either be bacterial, viral, bacterial artificial chromosomes, yeast artificial chromosomes, or artificial cosmids, depending on the host organism of choice. Plasmids can also be classified based on its function, such that there is a certain set of vectors that can be used if the goal of the experiment is to only propagate the gene, as is the case for this particular study. However, vectors that allow expression of particular gene into the corresponding protein are also available as well. The growth in the population of host cells containing the recombinant DNA molecule will also result to the replication of the gene clone. As added optimizing measure, aside from the G6Pase sequence and restriction sites, the vector also contains resistance genes against antibiotics. Thus, those that do not have the recombinant DNA molecule will be killed by antibiotics, allowing the transformed cells to flourish better. When the clone is needed for further analysis, it can easily be extracted out of the cell (U.S. Department of Energy Genome Program, 2009). It is important to note that the mouse genetic material is very similar to that of humans. Thus, a known mouse G6Pase sequence can be used in hybridization as the heterologous probe in identifying the human G6Pase (McClean, 1997). How will this work? Briefly, human DNA sample will be treated with restriction enzymes to cut the long strands into smaller fragments. After doing so, the treated extract will be run through gel electrophoresis to separate the smaller fragments by weight. Then, using the mouse gene attached to a dye as heterologous probe, the strand which contains the human G6Pase will be detected and isolated. Copies of this isolated gene can be amplified through polymerase chain reaction (PCR).